{"created":"2023-05-15T15:14:23.798520+00:00","id":31,"links":{},"metadata":{"_buckets":{"deposit":"29243c31-5ea1-43db-a017-0ef6198bb9fb"},"_deposit":{"created_by":10,"id":"31","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"31"},"status":"published"},"_oai":{"id":"oai:rchokkaido-cn.repo.nii.ac.jp:00000031","sets":["8:12"]},"author_link":["180","68","69","201"],"control_number":"31","item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-03","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"7","bibliographicPageStart":"1","bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"日本赤十字北海道看護大学紀要","bibliographic_titleLang":"ja"},{"bibliographic_title":"Journal of Japanese Red Cross Hokkaido College of Nursing","bibliographic_titleLang":"en"}]}]},"item_10002_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"pdf","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"本研究では、プリオン蛋白 (prion protein: PrP) の体内感染経路を検討するために、4種類のPrP (AB5058、B103、SAF32、8G8) 抗体を用いて正常マウスの大脳、副腎、脾臓、消化管、筋肉組織で免疫染色を行いPrPの局在を調べた。また、各抗体の至適条件を検索した。その結果、免疫染色の前処理として組織のオートクレーブ処理が不可欠であった。また、4種類のPrP抗体では、B103は中枢と末梢神経でよく反応し、SAF32は末梢神経とリンパ系でよく反応した。マウスの大脳では、PrP免疫活性が多くの神経細胞とその短い神経突起に見られた。副腎では、PrP免疫活性は髄質の多くのクロマフィン細胞と多数の神経線維に認められたが、皮質では認められなかった。脾臓では、PrP免疫活性が胚小節内の樹状細網細胞に強く認められた。消化管では、PrP免疫活性が平滑筋層内の神経細胞および神経線維に、また、粘膜上皮中のセロトニン免疫陽性細胞にも認められた。また消化管内のリンパ節では、PrP免疫活性が樹状細網細胞に強く認められた。舌筋、腹筋、心筋では、PrPの免疫活性が神経線維に認められた。これらの事実は、PrPの体内感染経路の可能性として、神経行性もしくは血行性があることを示唆するものである。","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本赤十字北海道看護大学","subitem_publisher_language":"ja"}]},"item_10002_relation_12":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"40017056757","subitem_relation_type_select":"NAID"}}]},"item_10002_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"日本赤十字北海道看護大学","subitem_rights_language":"ja"}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11558248","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13461745","subitem_source_identifier_type":"PISSN"}]},"item_10002_textarea_1":{"attribute_name":"抄録（英）","attribute_value_mlt":[{"subitem_textarea_language":"en","subitem_textarea_value":"For the aim of investigating the internal route of infection of prion proteis (PrP), this study examined the localizations of PrP in normal mouse organs (cerebrum, adrenal gland, spleen, intestine, and muscle tissues) by immunohistochemical method using four specific antisera (AB5058, B103, SAF32, and 8G8).  Optimum conditions for these antisera were also examined.  It was revealed that the autoclave treatment of the tissues was necessary for this immunohistochemical process.  B103 antisera showed immunoreactivities in the central and peripheral nerve tissues, while SAF32 showed immunoreactivities in the peripheral nerve tissues and in the lymphoid tissues.  In the cerebrum, the PrP immunoreactivities were seen in numerous neuronal cells and in the short nerve processes.  In the adrenal gland, PrP immunoreactivities were recognized in numerous chromaffin cells and nerve fibers in the medulla, but not in the cortex.  The PrP-immunoreactive follicular dendritic cells were localized in the lymphatic nodule of the spleen.  In the digestive tract, PrP immunoreactivities were seen in the neuronal cells and nerve fibers in the muscular layer, and in the serotonin-immunoreactivie endocrine cells in the mucosal epithelium.  Moreover, the strong PrP immunoreactivities were seen in the follicular dendritic cells in the intestinal lymph node.  The PrP-immunoreactive nerve fibers were recognized in the skeletal muscle of tongue and abdomen and myocardium.  These facts suggest the possibilities that the PrP presumably were infected by way of nerves and or blood vessels."}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{}],"affiliationNames":[{}]}],"creatorNames":[{"creatorName":"村林, 宏","creatorNameLang":"ja"},{"creatorName":"ムラバヤシ, ヒロシ","creatorNameLang":"ja-Kana"},{"creatorName":"Murabayashi, Hiroshi","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{}],"affiliationNames":[{}]}],"creatorNames":[{"creatorName":"関川, 三男","creatorNameLang":"ja"},{"creatorName":"セキカワ, ミツオ","creatorNameLang":"ja-Kana"},{"creatorName":"Sekikawa, Mitsuo","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{},{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{}],"affiliationNames":[{}]}],"creatorNames":[{"creatorName":"古岡, 秀文","creatorNameLang":"ja"},{"creatorName":"フルオカ, ヒデフミ","creatorNameLang":"ja-Kana"},{"creatorName":"Furuoka, Hidefumi","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{},{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{}],"affiliationNames":[{}]}],"creatorNames":[{"creatorName":"大森, 行雄","creatorNameLang":"ja"},{"creatorName":"オオモリ, ユキオ","creatorNameLang":"ja-Kana"},{"creatorName":"Oomori, Yukio","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-09-06"}],"displaytype":"detail","filename":"009_P001.pdf","filesize":[{"value":"4.9 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"マウスの神経、副腎、リンパ組織、消化管、および筋肉における正常プリオン蛋白の分布についての免疫組織化学的研究","url":"https://rchokkaido-cn.repo.nii.ac.jp/record/31/files/009_P001.pdf"},"version_id":"0c641d45-c0b3-4019-8fdd-93002b39f0d2"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"プリオン蛋白","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"マウス","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"神経","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"リンパ組織","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"副腎","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"[原著]マウスの神経、副腎、リンパ組織、消化管、および筋肉における正常プリオン蛋白の分布についての免疫組織化学的研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"[原著]マウスの神経、副腎、リンパ組織、消化管、および筋肉における正常プリオン蛋白の分布についての免疫組織化学的研究","subitem_title_language":"ja"},{"subitem_title":"[Original Articles]Immunohistochemical Study on the Localization of Normal Prion Protein in the Nerve, Adrenal Gland, Lymphatic Tissue, Intestine, and Skeletal Muscle of the Mouse","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"10","path":["12"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-09-06"},"publish_date":"2017-09-06","publish_status":"0","recid":"31","relation_version_is_last":true,"title":["[原著]マウスの神経、副腎、リンパ組織、消化管、および筋肉における正常プリオン蛋白の分布についての免疫組織化学的研究"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2023-07-13T07:48:22.392144+00:00"}