{"_buckets": {"deposit": "e0a3b027-bc5d-4750-95f1-dab6a12414d2"}, "_deposit": {"created_by": 10, "id": "21", "owners": [10], "pid": {"revision_id": 0, "type": "depid", "value": "21"}, "status": "published"}, "_oai": {"id": "oai:rchokkaido-cn.repo.nii.ac.jp:00000021", "sets": ["11"]}, "author_link": ["180", "140", "49", "97", "201"], "control_number": "21", "item_10002_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2008-03", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "38", "bibliographicPageStart": "35", "bibliographicVolumeNumber": "8", "bibliographic_titles": [{"bibliographic_title": "日本赤十字北海道看護大学紀要", "bibliographic_titleLang": "ja"}, {"bibliographic_title": "Journal of Japanese Red Cross Hokkaido College of Nursing", "bibliographic_titleLang": "en"}]}]}, "item_10002_description_19": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "pdf", "subitem_description_language": "ja", "subitem_description_type": "Other"}]}, "item_10002_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "本研究では、口腔内細菌の可視化を目的に核染色用の蛍光色素であるDiamidino-2-phenylindole(DAPI)を用いて口腔内細菌の蛍光染色を行った。検体中に含まれる口腔粘膜細胞や口腔内残査を除去するために直径6μm穴の濾紙で濾過し、その中から10μlをガラススライド上で乾燥させ、DAPI液を細菌の上に滴下し、30分反応させた。その結果、蛍光顕微鏡下でDAPI染色により細菌のDNAが染まり、球状あるいは連鎖状の細菌のみを可視化することができた。また成人5人による起床時の口腔内細菌数を計測した結果、各個人の相対的な口腔内細菌数の違いが明確になった。従って今回、DAPI蛍光染色による細菌の可視化条件が決められ、検体中の細菌の存在、数や形態を簡便にかつ短時間に顕微鏡下で観察および計測することが可能となった。今後この細菌のDAPI蛍光染色法は、医療、看護領域における細菌研究に応用出来るものと考える。", "subitem_description_language": "ja", "subitem_description_type": "Abstract"}]}, "item_10002_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "日本赤十字北海道看護大学", "subitem_publisher_language": "ja"}]}, "item_10002_relation_12": {"attribute_name": "論文ID（NAID）", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "40016313175", "subitem_relation_type_select": "NAID"}}]}, "item_10002_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "日本赤十字北海道看護大学", "subitem_rights_language": "ja"}]}, "item_10002_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA11558248", "subitem_source_identifier_type": "NCID"}]}, "item_10002_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "13461745", "subitem_source_identifier_type": "PISSN"}]}, "item_10002_version_type_20": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorAffiliations": [{"affiliationNameIdentifiers": [{"affiliationNameIdentifierScheme": "ISNI", "affiliationNameIdentifierURI": "http://www.isni.org/isni/"}], "affiliationNames": [{"affiliationNameLang": "ja"}]}], "creatorNames": [{"creatorName": "村林, 宏", "creatorNameLang": "ja"}, {"creatorName": "ムラバヤシ, ヒロシ", "creatorNameLang": "ja-Kana"}, {"creatorName": "Murabayashi, Hiroshi", "creatorNameLang": "en"}], "familyNames": [{"familyName": "村林", "familyNameLang": "ja"}, {"familyName": "ムラバヤシ", "familyNameLang": "ja-Kana"}, {"familyName": "Murabayashi", "familyNameLang": "en"}], "givenNames": [{"givenName": "宏", "givenNameLang": "ja"}, {"givenName": "ヒロシ", "givenNameLang": "ja-Kana"}, {"givenName": "Hiroshi", "givenNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "180", "nameIdentifierScheme": "WEKO"}]}, {"creatorAffiliations": [{"affiliationNameIdentifiers": [{"affiliationNameIdentifierScheme": "ISNI", "affiliationNameIdentifierURI": "http://www.isni.org/isni/"}], "affiliationNames": [{"affiliationNameLang": "ja"}]}], "creatorNames": [{"creatorName": "山本, 美紀", "creatorNameLang": "ja"}, {"creatorName": "ヤマモト, ミキ", "creatorNameLang": "ja-Kana"}, {"creatorName": "Yamamoto, Miki", "creatorNameLang": "en"}], "familyNames": [{"familyName": "山本", "familyNameLang": "ja"}, {"familyName": "ヤマモト", "familyNameLang": "ja-Kana"}, {"familyName": "Yamamoto", "familyNameLang": "en"}], "givenNames": [{"givenName": "美紀", "givenNameLang": "ja"}, {"givenName": "ミキ", "givenNameLang": "ja-Kana"}, {"givenName": "Miki", "givenNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "140", "nameIdentifierScheme": "WEKO"}]}, {"creatorAffiliations": [{"affiliationNameIdentifiers": [{"affiliationNameIdentifierScheme": "ISNI", "affiliationNameIdentifierURI": "http://www.isni.org/isni/"}], "affiliationNames": [{"affiliationNameLang": "ja"}]}], "creatorNames": [{"creatorName": "澤井, 幹樹", "creatorNameLang": "ja"}, {"creatorName": "サワイ, モトキ", "creatorNameLang": "ja-Kana"}, {"creatorName": "Sawai, Motoki", "creatorNameLang": "en"}], "familyNames": [{"familyName": "澤井", "familyNameLang": "ja"}, {"familyName": "サワイ", "familyNameLang": "ja-Kana"}, {"familyName": "Sawai", "familyNameLang": "en"}], "givenNames": [{"givenName": "幹樹", "givenNameLang": "ja"}, {"givenName": "モトキ", "givenNameLang": "ja-Kana"}, {"givenName": "Motoki", "givenNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "49", "nameIdentifierScheme": "WEKO"}]}, {"creatorAffiliations": [{"affiliationNameIdentifiers": [{"affiliationNameIdentifierScheme": "ISNI", "affiliationNameIdentifierURI": "http://www.isni.org/isni/"}], "affiliationNames": [{"affiliationNameLang": "ja"}]}], "creatorNames": [{"creatorName": "休波, 茂子", "creatorNameLang": "ja"}, {"creatorName": "ヤスナミ, シゲコ", "creatorNameLang": "ja-Kana"}, {"creatorName": "Yasunami, Shigeko", "creatorNameLang": "en"}], "familyNames": [{"familyName": "休波", "familyNameLang": "ja"}, {"familyName": "ヤスナミ", "familyNameLang": "ja-Kana"}, {"familyName": "Yasunami", "familyNameLang": "en"}], "givenNames": [{"givenName": "茂子", "givenNameLang": "ja"}, {"givenName": "シゲコ", "givenNameLang": "ja-Kana"}, {"givenName": "Shigeko", "givenNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "97", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000090274745", "nameIdentifierScheme": "CiNii ID", "nameIdentifierURI": "http://ci.nii.ac.jp/nrid/1000090274745"}]}, {"creatorAffiliations": [{"affiliationNameIdentifiers": [{"affiliationNameIdentifierScheme": "ISNI", "affiliationNameIdentifierURI": "http://www.isni.org/isni/"}], "affiliationNames": [{"affiliationNameLang": "ja"}]}], "creatorNames": [{"creatorName": "大森, 行雄", "creatorNameLang": "ja"}, {"creatorName": "オオモリ, ユキオ", "creatorNameLang": "ja-Kana"}, {"creatorName": "Oomori, Yukio", "creatorNameLang": "en"}], "familyNames": [{"familyName": "大森", "familyNameLang": "ja"}, {"familyName": "オオモリ", "familyNameLang": "ja-Kana"}, {"familyName": "Oomori", "familyNameLang": "en"}], "givenNames": [{"givenName": "行雄", "givenNameLang": "ja"}, {"givenName": "ユキオ", "givenNameLang": "ja-Kana"}, {"givenName": "Yukio", "givenNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "201", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-09-06"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "008_P035.pdf", "filesize": [{"value": "2.4 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 2400000.0, "url": {"label": "蛍光色素Diamidino-2-phenylindole(DAPI)を用いた口腔内細菌の可視化に関する基礎的研究", "url": "https://rchokkaido-cn.repo.nii.ac.jp/record/21/files/008_P035.pdf"}, "version_id": "a3fcd8e3-10f8-4bd0-8d44-63aff414830d"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Diamidino-2-phenylindole", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "DAPI", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "蛍光色素", "subitem_subject_language": "ja", "subitem_subject_scheme": "Other"}, {"subitem_subject": "細菌", "subitem_subject_language": "ja", "subitem_subject_scheme": "Other"}, {"subitem_subject": "DNA", "subitem_subject_language": "ja", "subitem_subject_scheme": "Other"}, {"subitem_subject": "可視化", "subitem_subject_language": "ja", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "[研究報告]蛍光色素Diamidino-2-phenylindole(DAPI)を用いた口腔内細菌の可視化に関する基礎的研究", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "[研究報告]蛍光色素Diamidino-2-phenylindole(DAPI)を用いた口腔内細菌の可視化に関する基礎的研究", "subitem_title_language": "ja"}, {"subitem_title": "[Research Articles]Fluorescence microscopic study of the visualization of bacteria in the human oral cavity by using fluorescence dye, diamidio-2-phenylindole (DAPI)", "subitem_title_language": "en"}]}, "item_type_id": "10002", "owner": "10", "path": ["11"], "permalink_uri": "https://rchokkaido-cn.repo.nii.ac.jp/records/21", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2017-09-06"}, "publish_date": "2017-09-06", "publish_status": "0", "recid": "21", "relation": {}, "relation_version_is_last": true, "title": ["[研究報告]蛍光色素Diamidino-2-phenylindole(DAPI)を用いた口腔内細菌の可視化に関する基礎的研究"], "weko_shared_id": -1}